Give any three reasons for the significance of transformation in plants
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Genetic transformation involves the integration of gene into genome by means other than fusion of gametes or somatic cells. The foreign gene (termed the "transgene") is incorporated into the host plant genome and stably inherited through future generations. This plant transformation approach is being used to generate plant processing trails, unachievable by conventional plant breeding, especially in case where there is no source of the desired trait in the gene pool.
In the gene of interest, the correct regulatory sequences are incorporated i.e. promoters and terminators, and then the DNA is transferred to the plant cell or tissue using a suitable vector. The gene of interest is attached to a selectable marker which allows selection for the presence of the transgene. Confirmation for the presence of inserted genes is generally tested by resistance to a specific antibiotic present in the medium.
In the gene of interest, the correct regulatory sequences are incorporated i.e. promoters and terminators, and then the DNA is transferred to the plant cell or tissue using a suitable vector. The gene of interest is attached to a selectable marker which allows selection for the presence of the transgene. Confirmation for the presence of inserted genes is generally tested by resistance to a specific antibiotic present in the medium.
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