Give two identification test for reducing sugar
Answers
To test for the presence of reducing sugars, a food sample is dissolved in boiling water. Next, a small amount of Benedict's reagent is added and the solution begins to cool. During the next four to 10 minutes, the solution should begin to change colors. If the color changes to blue, then no glucose is present. If a high amount of glucose is present, then the color change will progress to green, yellow, orange, red and then a dark red or brown.
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To carry out Fehling's test, the solution is diluted in water and warmed until fully dissolved. Next, Fehling's solution is added while stirring. If reducing sugars are present, the solution should begin to change colors as a rust or red colored precipitate forms. If reducing sugars are not present, the solution will remain blue or green.