give two methods of microbial assay of antibiotics
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▪Two general methods are usually employed, the cylinder-plate (or cup-plate) method and the turbidimetric (or tube assay) method.
The cylinder-plate method (Method A) depends upon diffusion of the antibiotic from a vertical cylinder through a solidified agar layer in a Petri dish or plate to an extent such that growth of the added micro-organism is prevented entirely in a zone around the cylinder containing a solution of the antibiotic. The turbidimetric method (Method B) depends upon the inhibition of growth of a microbial culture in a uniform solution of the antibiotic in a fluid medium that is favourable to its rapid growth in the absence of the antibiotic.
The assay is designed in such a way that the mathematical model on which the potency equation is based can be proved to be valid.
If a parallel-line model is chosen, the two log doseresponse lines of the preparation under examination and the standard preparation should be parallel; they should be rectilinear over the range of doses used in the calculation.
These conditions should be verified by validity tests for a given probability. Other mathematical models, such as the slope ratio method, may be used provided that proof of validity is demonstrated.
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^_^
▪Two general methods are usually employed, the cylinder-plate (or cup-plate) method and the turbidimetric (or tube assay) method.
The cylinder-plate method (Method A) depends upon diffusion of the antibiotic from a vertical cylinder through a solidified agar layer in a Petri dish or plate to an extent such that growth of the added micro-organism is prevented entirely in a zone around the cylinder containing a solution of the antibiotic. The turbidimetric method (Method B) depends upon the inhibition of growth of a microbial culture in a uniform solution of the antibiotic in a fluid medium that is favourable to its rapid growth in the absence of the antibiotic.
The assay is designed in such a way that the mathematical model on which the potency equation is based can be proved to be valid.
If a parallel-line model is chosen, the two log doseresponse lines of the preparation under examination and the standard preparation should be parallel; they should be rectilinear over the range of doses used in the calculation.
These conditions should be verified by validity tests for a given probability. Other mathematical models, such as the slope ratio method, may be used provided that proof of validity is demonstrated.
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