Question

Hey guys, need help
Why there is only one recognition site in cloning vectors?

Answer 1

vectors maintain a modified origin of replication that allows their replication within the host, and they contain a gene for antibiotic resistance which ensures that, following treatment with a high dose of antibiotic, all viable bacterial colonies will contain several copies of the plasmid.

Answer 2

The only and specific function of restriction sites is to be cut by a specific restriction enzyme to insert a target sequence in vector. Both the plasmid and insert (gene) must be digested with the same restriction enzymes in similar sites to produce cohesive and complementary ends.

Maybe because of the specific function and the specific restriction enzyme, there's only one restriction site in cloning vectors. Or maybe they extract it that way from microbes...

Mark it as the brainliest!

:)