how can we transfer therputical gene .....to host...in which medium
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Gene transfer technique is used very widely both in basic research and applied biology. The delivery of DNA into animal cells is a fundamental and established procedure. It has become an indispensable tool for gene cloning, the study of gene function and regulation and the production of small amounts of recombinant proteins for analysis and verification. Gene transfer experiment helps to express the introduced genetic construct (or transgene) in the recipient cells or to disrupt or inactivate particular endogenous genes (resulting in a loss of function. There are many applications of gene transfer like large-scale commercial production of recombinant antibodies and vaccines and gene medicine or gene therapy. They range from the use of mammalian and insect cell cultures to transfer of DNA into human patients for the correction or prevention of disease.
In the organisms or genetically modified whole animals created by Gene transfer every cell or a specific target population of cells carries a particular alteration. Such animals are used to study gene function and expression, model human diseases, produce recombinant proteins in their milk and other fluids, and to improve the quality of livestock herds and other domestic species. The technology has contributed to understand the functions of the many genes discovered in the genome projects (functional genomics). Examples of such experiments include systematic DNA-mediated mutagenesis and gene trap programs in the mouse and in the fruit fly, Drosophila melanogaster, genome-wide RNA interference experiments in the nematode, Caenorhabditis elegans, and novel protein interaction screens based on the yeast two hybrid system but performed using mammalian cells.
Genetic engineering of food is the science which involves deliberate modification of the genetic material of plants or animals. Introduction of DNA into plants is of great agricultural potential and medical importance. The gene transfer results can be transient and stable transfection.
Gene therapy can be defined as the deliberate transfer of DNA for therapeutic purposes. Gene transfer is one of the key factors in gene therapy, and it is one of the key purposes of the clone. Gene transfer can be targeted to somatic (body) or germ (egg and sperm) cells. In somatic gene transfer the recipient’s genome is changed, but the change will not be passed on to the next generation. In germline gene transfer, the parents’ egg and sperm cells are changed with the goal of passing on the changes to their offspring.
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Gene transfer technique is used very widely both in basic research and applied biology. The delivery of DNA into animal cells is a fundamental and established procedure. It has become an indispensable tool for gene cloning, the study of gene function and regulation and the production of small amounts of recombinant proteins for analysis and verification. Gene transfer experiment helps to express the introduced genetic construct (or transgene) in the recipient cells or to disrupt or inactivate particular endogenous genes (resulting in a loss of function. There are many applications of gene transfer like large-scale commercial production of recombinant antibodies and vaccines and gene medicine or gene therapy. They range from the use of mammalian and insect cell cultures to transfer of DNA into human patients for the correction or prevention of disease.
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