How is TCSPC FLIM-FRET used to measure distances?
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we present a novel multi dimensional time correlated single photon counting (TCSPC) technique to perform fluoresence lifetime imaging with a laser scanning microscope operated at a pixel dwell- time in the microsecond range the unsurpassed temporal accuracy of the approach combined with a high detection efficiency was applied to measure the fluorescent lifetime of enhanced cyan fluorescent protein (ECFP) in isolation and in tandem with EYFP enhance yellow fluorescent protein.
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