How many moles of analyte are present in 10.0 μM solution that occupies 1.0% of the length of a 60.0 cm × 25.0 μm capillary?
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Given data :
Analyte Present = 10.0μM
Solution that occupies = 1.0%
length = 60.0cm X 25.0μm
Capillary = 4.0 seconds
μapp. = 3.0 X 1 /(V.S)
a) The total a mount of injected analyte is the resultant of electroinjection and EOF and can e calculated from the equation.
N(A) = CA,A (μeof + ૪ μep) Eot
Mobility of analyse A,૪ is the ratio of the resistivity of the sample to BGS, ''E0'' -> is the electric field strength.
''t'' -> is the time of injection.
So we have to calculate N (A)
Molarity = 10 X 10^-6
Volume = (1/100) X 25 X 10^-6) X (60/100)
= 15 X 10^8
So moles = N(A) = 15 X 10^13
N(A) = 29.5 mol
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29.5
D option is correct
None of the above
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