how to generate electricity through soil
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Soil has been used to generate electrical power in microbial fuel cells (MFCs) and exhibited several potential applications. This study aimed to reveal the effect of soil properties on the generated electricity and the diversity of soil source exoelectrogenic bacteria. Seven soil samples were collected across China and packed into air-cathode MFCs to generate electricity over a 270 days period. The Fe(III)-reducing bacteria in soil were enriched and sequenced by Illumina pyrosequencing. Culturable strains of Fe(III)-reducing bacteria were isolated and identified phylogenetically. Their exoelectrogenic ability was evaluated by polarization measurement. The results showed that soils with higher organic carbon (OC) content but lower soil pH generated higher peak voltage and charge. The sequencing of Fe(III)-reducing bacteria showed that Clostridia were dominant in all soil samples. At the family level, Clostridiales Family XI incertae sedis were dominant in soils with lower OC content but higher pH (>8), while Clostridiaceae, Lachnospiraceae, and Planococcaceae were dominant in soils with higher OC content but lower pH. The isolated culturable strains were allied phylogenetically to 15 different species, of which 11 were Clostridium. The others were Robinsoniella peoriensis, Hydrogenoanaerobacterium saccharovorans, Eubacterium contortum, and Oscillibacter ruminantium. The maximum power density generated by the isolates in the MFCs ranged from 16.4 to 28.6 mW m-2. We concluded that soil OC content had the most important effect on power generation and that the Clostridiaceae were the dominant exoelectrogenic bacterial group in soil. This study might lead to the discovery of more soil source exoelectrogenic bacteria species
Procedure
Soil samples were collected from seven sites, which were located in the Inner Mongolia Autonomous Region (IM), Hebei Province (HB), Henan Province (HN), Jiangsu Province (JS), Jiangxi Province (JX), Fujian Province (FJ), and Guangxi Zhuang Autonomous Region (GX), respectively. The location information of the sampling sites is shown in Supplementary Table S1. Each site was planted with one dominant vegetation type. In each site, surface soil samples (0–20 cm) from three randomly selected plots (0.5 m × 0.5 m) were collected and mixed to represent a site, after removing the surface litter. After the soil samples were sieved and passed through a 2 mm diameter mesh, they were stored at 4°C for less than 2 weeks before a series of experiments, including Fe(III)-reducing bacteria enrichment, MFCs operation and soil property measurement.
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Soil has been used to generate electrical power in microbial fuel cells (MFCs) and exhibited several potential applications. This study aimed to reveal the effect of soil properties on the generated electricity and the diversity of soil source exoelectrogenic bacteria. Seven soil samples were collected across China and packed into air-cathode MFCs to generate electricity over a 270 days period. The Fe(III)-reducing bacteria in soil were enriched and sequenced by Illumina pyrosequencing. Culturable strains of Fe(III)-reducing bacteria were isolated and identified phylogenetically. Their exoelectrogenic ability was evaluated by polarization measurement. The results showed that soils with higher organic carbon (OC) content but lower soil pH generated higher peak voltage and charge. The sequencing of Fe(III)-reducing bacteria showed that Clostridia were dominant in all soil samples. At the family level, Clostridiales Family XI incertae sedis were dominant in soils with lower OC content but higher pH (>8), while Clostridiaceae, Lachnospiraceae, and Planococcaceae were dominant in soils with higher OC content but lower pH. The isolated culturable strains were allied phylogenetically to 15 different species, of which 11 were Clostridium. The others were Robinsoniella peoriensis, Hydrogenoanaerobacterium saccharovorans, Eubacterium contortum, and Oscillibacter ruminantium. The maximum power density generated by the isolates in the MFCs ranged from 16.4 to 28.6 mW m-2. We concluded that soil OC content had the most important effect on power generation and that the Clostridiaceae were the dominant exoelectrogenic bacterial group in soil. This study might lead to the discovery of more soil source exoelectrogenic bacteria species
Procedure
Soil samples were collected from seven sites, which were located in the Inner Mongolia Autonomous Region (IM), Hebei Province (HB), Henan Province (HN), Jiangsu Province (JS), Jiangxi Province (JX), Fujian Province (FJ), and Guangxi Zhuang Autonomous Region (GX), respectively. The location information of the sampling sites is shown in Supplementary Table S1. Each site was planted with one dominant vegetation type. In each site, surface soil samples (0–20 cm) from three randomly selected plots (0.5 m × 0.5 m) were collected and mixed to represent a site, after removing the surface litter. After the soil samples were sieved and passed through a 2 mm diameter mesh, they were stored at 4°C for less than 2 weeks before a series of experiments, including Fe(III)-reducing bacteria enrichment, MFCs operation and soil property measurement.
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chinglemba2:
could you please send me the procedure
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should i send here or there
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