How would you test the pH of soil in your laboratory
I want like lab active for example (aim,objectives,apparatus,procedure,inference) please send fast
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Experiment 6 Soil pH
Experimental Objective
The objective of this experiment is to determine the pH of soil and to classify the soil as sweet, sour, or neutral.
Pre Lab Questions
Read Introduction below.
What is the pH of soil?
What does the pH of soil depend on?
With your lab partner, devise a researchable question(s) prior to the designated lab session. You must also receive approval for your researchable question from Professor Schnitzer before the designated lab session.
Bring soil sample(s) to answer your researchable question(s).
Materials:
250 mL beaker, 100 mL graduated cylinder, 1000 mL volumetric flask, vial or small beaker
Vernier pH sensor/Lab Pro kit/Logger Pro (data collection)
Distilled (ultrapure) water
Soil sample (at least 50 grams)
Buffers (at least two)
CaCl2(s)
Timer or stopwatch
Spoon or stirring tool
Kimwipes
Pasteur pipet (2)
Introduction
When you think of pH, you probably think of acidic and basic solutions. But soil can be acidic or basic too. Soil pH, sometimes referred to as soil acidity, can be expressed using the pH scale. The pH scale ranges from 0 to 14. Soils with pH above 7 are basic or sweet. Soils with pH below 7 are acidic or sour. A soil with a pH of 7 is neither acidic nor basic – it is neutral.
The pH of soil is an important factor in determining which plants grow because it controls which nutrients are available for the plants to use. Three primary plant nutrients – nitrogen, phosphorus, and potassium – are required for healthy plant growth. Because plants need them in large quantities, they are called macronutrients. They are the main ingredients of most fertilizers that farmers and gardeners add to their soil. Other nutrients such as iron and manganese are also needed by plants, but only in very small amounts. These nutrients are called micronutrients.
The availability of these nutrients depends not only on the amount but also on the form that is present, on the rate they are released from the soil, and on the pH of the soil. In general, macronutrients are more available in soil with high pH and micronutrients are more available in soil with low pH. Figure 1 shows the effect of pH on the availability of nutrients in the soil.
Procedure
1. Prepare 0.01 M CaCl2
About 1 L of the solution should be made.
If preparing the solution, dissolve 1.11 g of solid calcium chloride in a 1000 mL volumetric flask with enough distilled water, then fill the flask until there is 1 L of CaCl2.
CaCl2 dissolves quickly when exposed to moisture, so weigh the solid CaCl2 as quickly as possible.
Dissolve the CaCl2 in the water.
Once the CaCl2 solution is prepared, set it aside.
2. Set up Lab Pro
Connect the power cord to the Lab Pro and plug it into the electrical outlet.
Connect the USB of the Lab Pro to the calculator (a beep indicates the Lab Pro is working).
Under Apps (blue button) choose 'Data Mate.'
Connect the pH sensor to the Lab Pro in channel 1.
Hit 'set up' (#1) then click 'calibrate' (#2).
Click on “calibrate now.”
Place pH sensor into one of the buffers and wait until for the voltage to stabilize.
Once the voltage is stabilized, hit enter and type the pH value under “point.”
Repeat steps 2g & 2h for the second buffer (clean probe in between uses).
When both pH values are entered, press “OK” (button #1). Place the pH sensors into the buffers again to see if the pH sensor is reading the pH value accurately.
3. Preparing Soil Mixture
Obtain soil from campus area, home, or from your professor.
Weigh 50 g of soil and place it into a 250 mL beaker.
Pour 100 mL of 0.01 M CaCl2 solution into the soil in the beaker.
Mix the soil and the CaCl2 thoroughly.
Have a timer or stopwatch ready so that the soil mixture can be stirred once every 3 minutes for 15 minutes.
Once 15 minutes is over, let the soil mixture sit so that it will settle into 2 layers. There will be a top water layer and a bottom soil layer.
The soil mixture should sit for at least 5 minutes but if the top layer of water is not clear enough, then give the mixture another couple of minutes to settle.
4. Measuring pH value
Rinse the pH sensor with distilled water before using it to measure the pH. What is the pH of ultrapure/distilled water? What is the pH of the CaCl2 solution?
When the mixture has settled, use a Kimwipe as a filter in a pipet. To do this, tear off a piece of Kimwipe, and stick it about half way down the pipet. Use a second pipet to transfer the top layer of the soil mixture through the filtered pipet into a vile (or beaker). This is called the “Kimwipe extraction method.”
Use the pH probe to measure the pH of the top layer of the soil mixture in the vile (make sure the glass tip of the probe is submerged in the solution).
Stir the top layer of water gently and wait for the pH reading to stabilize.
When the pH reading is stabilized, record its pH value.
Experimental Objective
The objective of this experiment is to determine the pH of soil and to classify the soil as sweet, sour, or neutral.
Pre Lab Questions
Read Introduction below.
What is the pH of soil?
What does the pH of soil depend on?
With your lab partner, devise a researchable question(s) prior to the designated lab session. You must also receive approval for your researchable question from Professor Schnitzer before the designated lab session.
Bring soil sample(s) to answer your researchable question(s).
Materials:
250 mL beaker, 100 mL graduated cylinder, 1000 mL volumetric flask, vial or small beaker
Vernier pH sensor/Lab Pro kit/Logger Pro (data collection)
Distilled (ultrapure) water
Soil sample (at least 50 grams)
Buffers (at least two)
CaCl2(s)
Timer or stopwatch
Spoon or stirring tool
Kimwipes
Pasteur pipet (2)
Introduction
When you think of pH, you probably think of acidic and basic solutions. But soil can be acidic or basic too. Soil pH, sometimes referred to as soil acidity, can be expressed using the pH scale. The pH scale ranges from 0 to 14. Soils with pH above 7 are basic or sweet. Soils with pH below 7 are acidic or sour. A soil with a pH of 7 is neither acidic nor basic – it is neutral.
The pH of soil is an important factor in determining which plants grow because it controls which nutrients are available for the plants to use. Three primary plant nutrients – nitrogen, phosphorus, and potassium – are required for healthy plant growth. Because plants need them in large quantities, they are called macronutrients. They are the main ingredients of most fertilizers that farmers and gardeners add to their soil. Other nutrients such as iron and manganese are also needed by plants, but only in very small amounts. These nutrients are called micronutrients.
The availability of these nutrients depends not only on the amount but also on the form that is present, on the rate they are released from the soil, and on the pH of the soil. In general, macronutrients are more available in soil with high pH and micronutrients are more available in soil with low pH. Figure 1 shows the effect of pH on the availability of nutrients in the soil.
Procedure
1. Prepare 0.01 M CaCl2
About 1 L of the solution should be made.
If preparing the solution, dissolve 1.11 g of solid calcium chloride in a 1000 mL volumetric flask with enough distilled water, then fill the flask until there is 1 L of CaCl2.
CaCl2 dissolves quickly when exposed to moisture, so weigh the solid CaCl2 as quickly as possible.
Dissolve the CaCl2 in the water.
Once the CaCl2 solution is prepared, set it aside.
2. Set up Lab Pro
Connect the power cord to the Lab Pro and plug it into the electrical outlet.
Connect the USB of the Lab Pro to the calculator (a beep indicates the Lab Pro is working).
Under Apps (blue button) choose 'Data Mate.'
Connect the pH sensor to the Lab Pro in channel 1.
Hit 'set up' (#1) then click 'calibrate' (#2).
Click on “calibrate now.”
Place pH sensor into one of the buffers and wait until for the voltage to stabilize.
Once the voltage is stabilized, hit enter and type the pH value under “point.”
Repeat steps 2g & 2h for the second buffer (clean probe in between uses).
When both pH values are entered, press “OK” (button #1). Place the pH sensors into the buffers again to see if the pH sensor is reading the pH value accurately.
3. Preparing Soil Mixture
Obtain soil from campus area, home, or from your professor.
Weigh 50 g of soil and place it into a 250 mL beaker.
Pour 100 mL of 0.01 M CaCl2 solution into the soil in the beaker.
Mix the soil and the CaCl2 thoroughly.
Have a timer or stopwatch ready so that the soil mixture can be stirred once every 3 minutes for 15 minutes.
Once 15 minutes is over, let the soil mixture sit so that it will settle into 2 layers. There will be a top water layer and a bottom soil layer.
The soil mixture should sit for at least 5 minutes but if the top layer of water is not clear enough, then give the mixture another couple of minutes to settle.
4. Measuring pH value
Rinse the pH sensor with distilled water before using it to measure the pH. What is the pH of ultrapure/distilled water? What is the pH of the CaCl2 solution?
When the mixture has settled, use a Kimwipe as a filter in a pipet. To do this, tear off a piece of Kimwipe, and stick it about half way down the pipet. Use a second pipet to transfer the top layer of the soil mixture through the filtered pipet into a vile (or beaker). This is called the “Kimwipe extraction method.”
Use the pH probe to measure the pH of the top layer of the soil mixture in the vile (make sure the glass tip of the probe is submerged in the solution).
Stir the top layer of water gently and wait for the pH reading to stabilize.
When the pH reading is stabilized, record its pH value.
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