Question

In phase contrast microscopy, the rate at which light enters through object is

Answer 1

Answer:

Commonly used chemical fixatives include osmic acid and mainly glutaraldehyde. But for light microscopy heat is the most commonly used fixative. Explanation: Phase contrast microscopy is based on the fact that the rate at which light travels through objects is inversely related to their refractive indices.

Answer 2

Answer:

In phase contrast microscopy the rate at which light enters through the object is inversely proportional to their refractive indices.

Explanation:

• The phase contrast microscopy creates phase change in the direction of the light. It is induced by the thickness, refractive index of different regions of the object.
• The phase-contrast microscope has an annular stop that forms a hollow cone of light.
• When the light from the condenser lens strikes the specimen, the wavelength of the light is retarded to its quarter due to the difference in the refractive index of the specimen to the background.