Mention any three vector -less methods that are used to introduce recombinant DNA into a components host cells?
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Cells have membranes that prevent DNA from simply diffusing in or out. This is the initial barrier that scientists must overcome in order to insert foreign DNA into a cell. The four ways of accomplishing this goal are transduction, transformation, transfection and injection.
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The three ways used to introduce the desired DNA segment into a bacterial cell in recombinant technology experiments are
1.Microinjection
2.Heat shocking method
3.Gene-gun or biolistics method.
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