mention the steps involved in cloning of foreign DNA in cosmid vector
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The cloning of a foreign DNA in cosmid vector involves the following steps: (1) ligation of the foreign DNA between two cos sites; (2) making a concatemeric DNA; (3) in vitro packaging to introduce the DNA into the phage head to form the matured phage particle; and (4) introduction of the cloned DNA into E.
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The cloning of a foreign DNA in cosmid vector involves the following steps: (1) ligation of the foreign DNA between two cos sites; (2) making a concatemeric DNA; (3) in vitro packaging to introduce the DNA into the phage head to form the matured phage particle; and (4) introduction of the cloned DNA into E.
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