Preparation of phenol chloroform for dna extraction
Answers
Phenol-chloroform DNA extraction method is a well known and widely accepted DNA extraction method since long. We had discussed the history and outline of different types of DNA extraction method in our previous article.
Please read the article here,
different types of DNA extraction method
Phenol, chloroform and isoamyl alcohol are top three ingredients which is used in liquid-liquid DNA extraction. The solubility is an important property of any biological molecules. Different molecules can be separated on the basis of their solubility in solution or water.
The liquid-liquid DNA extraction method separates DNA molecule on the basis of their solubility in immiscible solutions and this is the basic principle of Phenol chloroform DNA extraction method.
In this article, we will discuss the phenol-chloroform DNA extraction method, preparation of phenol-chloroform and isoamyl alcohol, role of each chemical, preparation of phenol for DNA extraction and finally my ultimate guide for phenol-chloroform DNA extraction method.
The content of the article,
The basic principle of phenol-chloroform DNA extraction
Role of each chemical in Phenol chloroform DNA extraction
Preparation of phenol for DNA extraction (saturated)
General protocol of phenol-chloroform DNA extraction method
Preparation of each chemical
Advantages of the phenol-chloroform DNA extraction method
Limitations of the phenol-chloroform DNA extraction method
My ultimate guide for the phenol-chloroform DNA extraction method