Question

Q. In vitro amplification of nucleic acids can be carried out by
1) Norther blotting
2) RAPD
3) HPLC
4) PAGE

#ANSWER THE QUESTION WITH PROPER EXPLANATION

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Answer 1

2 RAPD....RAPD is a polymerase chain reaction (PCR) technique in which a random primer of >10 nucleotides is used as a template to generate DNA fragments which are then separated by gel electrophoresis....Polymerase chain reaction (PCR) was the first nucleic acid amplification method

A-Northern blotting- where isolated RNA separated

B-HPLC-Chromatography technique

D-PAGE-Electrophoresis

So the answer is PCR,i.e,RAPD

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Answer 2

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Option 2) RAPD.

REASONS :-

☆ During the amplification of nucleic acids in vitro, we can see that the factors that are associated with the synthesis of RNA .

☆ The full form of RAPD is Random Amplification of Polymorphic DNA.

☆ RAPD is pronounced as Rapid.

☆ RAPDs are fragments of DNA that are amplified by PCR ( Polymerise Chain Reaction) which is the most commonly accepted technique in laboratories.

☆ They are able to amplify 1-10 sites simultaneously.

☆ Reproductivity of PCR-RAPD can be done on two or three different days.

☆ It is used for the identification of genetic variation.

☆ RAPD is a type of PCR while the amplified DNA segments are in random at the same locus.

☆ The common and important function of RAPD is that it binds to the nonspecific DNA sites and amplifies it.

☆ So it is easier to amplify nucleic acids in vitro by RAPD. ( RAPD-PCR bond ).

MORE INFORMATION :-

○ Northern Blotting

It is the tecnique to analyse the abundance of RNA.

○ HPLC

HPLC is High-performance liquid chromatography used in analytical chemistry.

○ PAGE

Polyacrylamide gel electrophoresis is the tecnique of seperating biological macromolecules.