Question

Recombinant insulin is produced at 100 mg/L by E. coli at a cell concentration of 1 g/L. Calculate the volume of reactor (size of the fermentor) needed to produce 1 Kilogram of insulin in the following conditions:
(a) When the cell concentration is 1 g/L and insulin production is 100 mg /L.
(b) When the cell concentration is 50 g/L and insulin production is 100 mg /L.

Answer 1

Insulin is a well-characterized peptide that can be produced by recombinant DNA technology for human therapeutic use. ... Analytical systems for insulin separation are principally based on reversed-phase chromatography, which resolves the deamidation product(s) (desamido insulin) of insulin, proinsulin, and insulin.