Question

Role of different restriction enzyme in pbr 322 vector

Answer 1

pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. it was named after researchers who constructed it. The p stands for "plasmid," and BR for "Bolivar" and "Rodriguez."

Answer 2

It is the first plasmid vector widely used for cloning DNA in E. coil

It has ori site that initiates the replication of plasmid DNA

rop codes for protein involved in the replication of the plasmid.

pBR-322 showing restriction sites for the enzymes Hind III, Eco RI, Bam HI, Sal I, Pvu II, Pst I, Cla l etc

It has antibiotics resistance genes like

$amp {}^{R} and \: tet {}^{R}$

Which are resistantce to the antibiotics like Ampicillan, and Tetracycline respectively.

Such antibiotic resistance genes are selectable markers used to identify recombinants from non recombinants

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