Question

Short sequence of dna used for identification of complimentary sequence in a sample are known as

Answer 1

The primer's sequence is complementary to the first piece of target DNA, which means that the primer and the DNA target bind with each other.

Answer 2

Answer: Short sequence of DNA used for identification of complementary sequence in a sample are known as " DNA probes"

Explanation:

A probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome.

The probe is placed into contact with the sample under conditions that allow the probe sequence to hybridize with its complementary sequence.

It is used to detect a specific DNA fragment in q(quantitative)PCR

There are many types of DNA probes:-

1. Gene-specific probes ( they are gene-dependent probes like a specific mRNA is used )
2. Oligonucleotide probes
3. Polymorphic probes

Role:  

• Diagnosis of infectious diseases includes rapid detection and identification of infectious agents.

• The ability to screen selected specimens using batteries of probes.

• And the detection of nonviable or difficult-to-culture organisms.

Autoradiography is used to detect the hybridization, however, the radiolabelled molecules are not safer to use.