Significance of salting in and salting out
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Salting in. Salting in refers to the effect where increasing the ionic strength of a solution increases the solubility of some solute (such as a protein). ... But at high concentrations of salt, the solubility of the proteins drop sharply (salting out).
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- "Salting in" refers to the observation that at solutions of low salt concentrations, the solubility of a protein increases with the addition of salt.
- By contrast, "salting out" requires high salt concentration for the precipitation of the protein.
- There are two ways of "salting out".
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