Biology, asked by bhoomika6294, 1 year ago

Specify the role of alkaline phosphatase in cloning.

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Answered by Anonymous

Typical uses in the lab for alkaline phosphatases include removing phosphate monoesters to prevent self-ligation, which is undesirable during plasmid DNA cloning. ... This phosphatase is easily inactivated by heat, a useful feature in some applications. Calf-intestinal alkaline phosphatase (CIP)

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