Biology, asked by kailaskambale040, 8 months ago

test for fat in observation and result​

Answers

Answered by sonusalesagencies
6

Answer:

here ya go

Explanation:

Aim: To test the presence of fat in the given food item

Things required:One piece of paper,food item

method

1. take small quantity of food item in a piece of paper

2. fold the paper and crush it

3. record your observation

observation:we can only see patches on paper

result: this activity proved that fat is present in the given food item

diagram:

Attachments:
Answered by atiya12
1

The following are the test to identify the presence of fats and oils.

Solubility test

Solubility testTranslucent spot test

Solubility testTranslucent spot testAcrolein test

Solubility testTranslucent spot testAcrolein testBaudouin test

Solubility testTranslucent spot testAcrolein testBaudouin testHuble’s test

(a) Solubility Test:

Fats are soluble in organic solvents like chloroform, alcohol etc. It is insoluble in water. So if the given sample forms an oily layer above the surface of the water then fat is present. Partially soluble in alcohol and fully soluble in chloroform then the presence of fat is confirmed.

Note: If the sample is miscible with chloroform and immiscible with water the fat presence is confirmed.

(b) Translucent Spot Test:

In this test the given sample to be tested is rubbed between the folds of filter paper. The appearance of translucent spot confirms the presence of fats in the given sample.

Note: If there is presence of translucent spot then the presence of fats is confirmed.

(c) Acrolein Test:

Fats and oils when heated with some crystals of potassium bisulfite KHSO4 in a test tube. A pungent irritating odor or smell of acrolein confirms the presence of fat or oil.

Note: If there is a pungent irritating odor then the presence of fats or oil is confirmed.

(d) Baudouin Test:

Baudouin test is applied to distinguish between the desi ghee and vanaspati ghee. Vanaspati ghee contains 5% sesame oil. Pure ghee does not contain sesame oil. Fats and oils are treated with 5ml of concentrated hydrochloric acid and 2% furfural solution in alcohol. After 5-10 minutes rose red colour appearance shows the presence of sesame oil in the given sample.

Note: This test is used to find out whether the given sample of desi ghee contains vanaspati or not.

(e) Huble’s Test:

This test is used to know the degree of unsaturation in the given sample. Oils on reaction with Huble’s reagent fads the violet colour of iodine then it is unsaturated and if the colour persists then the given fat or oil is saturated.

Note: In this test various oils can be compared on the basis of unsaturation.

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