the plasmid generally used for the production of recombinant insulin is
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ᴛʜᴇ ɢᴇɴᴇ ᴏғ ɪɴᴛᴇʀᴇsᴛ (ɪᴇ. ɪɴsᴜʟɪɴ ᴄᴏᴅɪɴɢ ɢᴇɴᴇ) ɪs ɪsᴏʟᴀᴛᴇᴅ ғʀᴏᴍ β-ᴄᴇʟʟ ᴀɴᴅ ɪɴsᴇʀᴛᴇᴅ ɪɴ ᴏᴘᴇɴᴇᴅ ᴘʟᴀsᴍɪᴅ. ᴛʜɪs ʀᴇᴄᴏᴍʙɪɴᴇᴅ ᴘʟᴀsᴍɪᴅ ɪs ɪɴsᴇʀᴛᴇᴅ ɪɴᴛᴏ sᴜɪᴛᴀʙʟᴇ ʜᴏsᴛ ᴄᴇʟʟ (ɪᴇ ᴇ. ᴄᴏʟɪ) ᴀɴᴅ ɴᴏᴡ ᴛʜɪs ʀᴇᴄᴏᴍʙɪɴᴇᴅ ʜᴏsᴛ ᴄᴇʟʟ sᴛᴀʀᴛs ᴘʀᴏᴅᴜᴄɪɴɢ ɪɴsᴜʟɪɴ ʜᴏʀᴍᴏɴᴇ.
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Insulin produced by recombinant DNA technology.
At first suitable vector (plasmid) is isolated from E. coli and then it is cut open by restriction endonuclease enzyme. ... This recombined plasmid is inserted into suitable host cell (ie E. coli) and now this recombined host cell starts producing insulin hormone.
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