Question

The purpose of secondary filter in florescence spectroscopy

Answer 1

Answer:

The primary filter selects short wavelength radiation capable of causing excitation of the test substance, while the secondary filter is normally a sharp cut-off filter that allows the longer wavelength fluorescence to be transmitted but blocks the scattered excitated radiation.

Explanation:

Answer 2

The purpose of secondary filter in florescence spectroscopy allows only emission radiation.

• The primary filter is capable of short wavelength radiation for causing excitation of the test, whereas secondary filter is usually sharp cut-off filter which allows longer wavelength fluorescence to transmit but would block the scattered excitation radiations.
• Secondary filter also allows emission radiations only.
• Secondary filters isolate desired emitted or fluorescent light.