Question

two dimensional chromatography is specially suitable when Rf values are a. very close b. greatly different c. nearly one​

Answer 1

c) nearly one is the correct option

Answer 2

The correct option is nearly one.

Explanation:

For an example of two-dimensional chromotography

Objective:

To study the phenols, flavonoids, saponin profile of the medicinal plant Cassytha filiformis (C. filiformis) using high-performance thin-layer chromatography (HPTLC).

Methods:

• The extracts were tested to determine the presence of various phytochemicals like alkaloids, phenolic compounds, flavonoids, carbohydrates, glycosides, saponins, terpenoids, tannins, fixed oils, fats and protein and amino acids (Harborne and Harborne, 1998).
• HPTLC studies were carried out by Harborne and Wagner et al method. Different compositions of the mobile phase for HPTLC analysis were tested in order to obtain high resolution and reproducible peaks.

Results:

• The results of the preliminary phytochemical studies confirm the presence of phenols, alkaloids, carbohydrates, saponins, flavonoids, terpenoids, and tannins in the methanolic extracts of C. filiformis.
• The methanolic extracts of C. filiformis displayed the presence of 13 types of phenolic substances with 13 different Rf values ranging from 0.01 to 0.96.
• The results illustrated the presence of 9 different types of flavonoids with 9 different Rf values ranging from 0.01 to 0.97.
• The results of the HPTLC analysis of saponins demonstrated the presence of 11 different types of saponins with 11 different Rf values ranging from 0.04 to 0.92.

Conclusions:

• In the present study, we observed the phenols, flavonoids, saponin profile of the medicinal plant C. filiformis using high-performance thin-layer chromatography (HPTLC).
• Hence it was concluded that the phenolic compounds present in the methonolic extract could be responsible for antioxidant activities.
• Plant-derived antioxidants, especially phenols and flavonoids, have been described to have various properties like anticancer, antiaging, and prevention of cardiovascular diseases.
• Further, separation and characterization of the bioactive compound from the plant are to be evaluated and reported in near future.