Question

Upto which passage cancer cell lines should be cultured

Answer 1

For cancer (immortal cells) passage numbers generally do not affect for treatment with drugs or any other agents. We have received cell lines (e.g. MCF-7 breast cancer cells and MDAMB-468) around three years back from ATCC and continuing using them. However, for primary cell culture (e.g. endothelial cells from umbilical cord) low passage number (4-5 passages) is highly important. For example estrogen receptors are gradually decreased with increasing passage number of HUVEC and after 15-16 passages , no estrogen receptors expressed.
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