what are the steps in PCR cycle??????
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➡ The steps of PCR cycle is
Denaturation
Annealing
extension
TAQ polymerase is used ..
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HI LEARNER,
Here is your answer,
PCR ( Polymerase Chain Reaction ) involves the steps of denaturation , annealing and primer extension .
In the first step , i.e., denaturation , the two DNA strands are departed by heating to 94 degree celcius which breaks the hydrogen bonding in between , so that each strand would act as a template for the synthesis of new strand . Such separation is essential in case of any replication . Here heat aids in separation through denaturation.
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