Question

What is DNA finger printing? Describe various steps involved in the same.

Answer 1

Dear Student,

● DNA fingerprinting -

• Method of isolating and identifying specific base pairs of DNA which is unique for every individual.

● Steps of DNA fingerprinting -

1. Isolation of DNA
2. Cutting of DNA by restriction endonuclease
3. Gel electrophoresis
4. Transfer of DNA on nitrocellulose membrane
5. Labelling of probe
6. Hybridization
7. Autoradiography
8. Interpretation of band pattern

◆ Applications of DNA fingerprinting -

1. DNA fingerprinting is used for detection of inherited disorders.
2. It is used for investigation of crime scene.
3. It is used to solve paternity disputes.
4. Monitoring of transplants is done by DNA fingerprinting.
5. It is used in anthropological studies.

Thanks dear. Hope this helps you...

Answer 2

DNA fingerprint:

DNA fingerprinting is the process of finding the evidence on the culprit who had been involved in criminal cases.  

Explanation:

• In this process the DNA fingerprint on the object of evidence is captured and then processes in the lab for DNA testing.
• Hence the DNA from the evidence and suspect (criminal) is kept for matching by running the gel, if the match is positive then the suspect is proved to be in a criminal case.