Question

What is hplc method development?

Answer 1

Hii friend,

# HPLC method development-

HPLC stands for High Performance Liquid Chromatography.

# Steps involved in HPLC method development include-

- 1) information on sample

- 2) define seperation goals

- 3) special procedure requirements sample pretreatment if necessary

- 4) detector selection and testing

- 5) separation condition optimization

- 6) check for problems or special procedure requirements

- 7) recovery of purified material

- 8) qualitative calibration

- 9) method validation for release to labs

Hope you understand...

Answer 2

HPLC is high performance liquid chromatography that is used to separate components from a mixture based on the pressure applied to the column and the interaction of components of mixture with the adsorbent material. Steps involved in method development of Hplc include:  

1. Selection of method and the initial instruments for HPLC

2. Optimization of the system

3. Selection of optimal conditions for performing the procedure of HPLC

4. Optimization of the parameters of the system

5. Validation of method