What is liquid culture? What's its advantages and disadvantages? Its uses?
Answers
Nutrient broth is typically made of a powdered beef extract that contains peptones (broken down proteins). The powder is dissolved in water, put in test tubes, and sterilized.
Broth is convenient, as most bacteria will grown in this type of medium, even those with widely different aerotolerances (oxygen requirements).
Different Types of Oxygen Requirements of Bacteria
Unlike animals, bacteria do not all require oxygen. Some bacteria are poisoned by oxygen, others can take it or leave it. Liquid broth allows bacteria to grow at varying oxygen levels, since the oxygen available decreases as the depth of the broth increases. See the test tube diagram below for an illustration of the growth patterns of microbes with different oxygen requirements:
Liquid culture is a combination of a sterilized nutritious solution, usually a mixture of water and various types of sugars inoculated with mycelium or fungus spores.
Advantages of liquid culture- Liquid culture is suitable to use in test tubes to grow bacteria, and may reveal information about the oxygen(O₂) requirements of growing bacteria within.
Disadvantages of liquid culture- Bacteria that grow in liquid media may not have specific characteristics.Difficult to isolate different types of bacteria from mixed populations.Bacteria grow diffusely in liquids they produce discrete visible growth in solid media.
A major advantage of using liquid culture is that it makes it so easy to inoculate jars. We don't need to open the jar's lid, we just inject on jars in order to create master spawn through the self - healing injection port.