Science, asked by sokamen7dranj, 1 year ago

what is melting temperature of primers

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Answered by sriramsundar
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. Primer Melting Temperature: Primer Melting Temperature (Tm) by definition is the temperature at which one half of the DNA duplex will dissociate to become single stranded and indicates the duplex stability. Primers with melting temperatures in the range of 52-58 oC generally produce the best results.
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