Question

What is pulse chase experiment?

Answer 1

In biochemistry and molecular biology, a pulse chase analysis is a method for examining a cellular process occurring over time by successively exposing the cells to a labeled compound and then to the same compound in an unlabeled form.

Answer 2

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➮ ᴘᴜʟsᴇ-ᴄʜᴀsᴇ ᴀɴᴀʟʏsɪs ɪs ᴀ ᴍᴇᴛʜᴏᴅ ғᴏʀ ᴇxᴀᴍɪɴɪɴɢ ᴀ ᴄᴇʟʟᴜʟᴀʀ ᴘʀᴏᴄᴇss ᴏᴄᴄᴜʀʀɪɴɢ ᴏᴠᴇʀ ᴛɪᴍᴇ ʙʏ sᴜᴄᴄᴇssɪᴠᴇʟʏ ᴇxᴘᴏsɪɴɢ ᴛʜᴇ ᴄᴇʟʟs ᴛᴏ ᴀ ʟᴀʙᴇʟᴇᴅ ᴄᴏᴍᴘᴏᴜɴᴅ (ᴘᴜʟsᴇ) ᴀɴᴅ ᴛʜᴇɴ ᴛᴏ ᴛʜᴇ sᴀᴍᴇ ᴄᴏᴍᴘᴏᴜɴᴅ ɪɴ ᴀɴ ᴜɴʟᴀʙᴇʟᴇᴅ ғᴏʀᴍ (ᴄʜᴀsᴇ).

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☞ɪɴ ᴀ ᴘᴜʟsᴇ ᴄʜᴀsᴇ ᴇxᴘᴇʀɪᴍᴇɴᴛ, ᴄᴇʟʟs ᴀʀᴇ ɢʀᴏᴡɴ ɪɴ ᴍᴇᴅɪᴜᴍ ᴀɴᴅ ᴇxᴘᴏsᴇᴅ ғᴏʀ ᴀ sʜᴏʀᴛ ᴛɪᴍᴇ ᴘᴇʀɪᴏᴅ ᴛᴏ ᴀ ʟᴀʀɢᴇ ᴀᴍᴏᴜɴᴛ ᴏғ ʀᴀᴅɪᴏᴀᴄᴛɪᴠᴇ ᴀᴍɪɴᴏ ᴀᴄɪᴅ. ᴛʜɪs ɪs ᴄᴀʟʟᴇᴅ ᴛʜᴇ ᴘᴜʟsᴇ, ᴀɴᴅ ᴅᴜʀɪɴɢ ᴛʜɪs ᴛɪᴍᴇ ᴘᴇʀɪᴏᴅ, ᴀɴʏ ɴᴇᴡʟʏ sʏɴᴛʜᴇsɪᴢᴇᴅ ᴘʀᴏᴛᴇɪɴs ᴡɪʟʟ ɪɴᴄᴏʀᴘᴏʀᴀᴛᴇ ᴛʜᴇ ʀᴀᴅɪᴏᴀᴄᴛɪᴠᴇʟʏ ᴀᴍɪɴᴏ ᴀᴄɪᴅ.