what is sterilization why is it essential in tissue culture
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Plant tissue or cell culture requires various combinations of nutrients, minerals, plant growth substances, vitamins and sugars (as carbon source). ... Thus, it is imperative to use sterile techniques for all in vitro plant culture manipulations.
Sterilization refers to any process that eliminates, removes, kills, or deactivates all forms of life and other biological agents present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media.
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