Question

What is the recognition sequence of Hind II restriction endonuclease?

Answer 1

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$\mathfrak{\green{Answer:}}$HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis.
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Answer 2

Answer:

Hind II restriction endonuclease recognizes the double-stranded sequence GTYRAC in a DNA strand and cleaves after the Y-3 position in the sequence.

Explanation:

It is a Type II restriction enzyme which brings about endonucleolytic cleavage of a DNA to generate double-stranded fragments with terminal 5'-phosphates in them.

Type II restriction enzymes cut DNA at specific positions near or within the recognition sequences that produces restriction fragments and thereby generating distinct gel banding patterns. They are used for routine DNA analysis and also gene cloning.