Question

What is the role of resuspension buffer in plasmid isolation?

Answer 1

Answer:

Resuspension buffer (solution I) is used for the isolation of plasmid DNA by alkaline lysis method. ... The purpose of resuspension buffer is to provide an optimal starting pH (pH 8.0) and an ideal condition for subsequent lysis.

Answer 2

Resuspension buffer helps in denaturing of DNA.

Explanation:

• Resuspension buffer  is a basic pH Tris buffer often having a basic pH of 8.0.
• Resuspension buffer helps in isolation of plasmid DNA by alkaline lysis method as the DNA is acidic in nature.
• It provides a minimal starting point of 8.0 pH for alkaline lysis to take place which is an optimal pH required for subsequent lysis to take place.
• EDTA is also used to prevent the damage of DNA by DNAses by chelating divalent cations.