Question

Which of the following are functions of water in the culture medium?
A
nutrients must be in aqueous solution
B
cofactor of enzymes
C
provides resistance to sudden transient temperature change
D both a and c​

Answer 1

Answer:

Water is used in many steps of the tissue or cell culture process. It is the main component of buffers and media, it is used for the dissolution of additives and drugs, and for rinsing bioreactors, plasticware and glassware. Thus, water quality may play an important role in cell culture experimental outcomes.

Answer 2

Answer:

Option D. both A and C

Explanation:

• A culture medium or growth medium is a solid, liquid, or semi-solid medium, designed to support the growth of the microbial population or cells via cell proliferation or small plants like the moss named Physcomitrella patens.

• Water in the culture medium acts as a chemical reactant, nutrients must also be present in the aqueous solution which provides resistance to sudden temperature variations.

• Water is used in many steps of the tissue or cell culture process. Water is the essential component of buffers and growth media preparation.

• It is used for the dissolution of various additives and drugs, and for rinsing bioreactors, equipment, plasticware and glassware etc. Thus, water quality plays an important role in cell culture experimental outcomes.

Thus, option d. both statements a and c. is the most correct and appropriate answer that supports the functions of water in the culture medium.

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