Question

Which of the following is immobilized on the microtiter well in sandwich ELISA?
capture antibody
Free antibody
1 ab
2 ab​

Answer 1

Answer:

2

Explanation:

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Answer 2

The Capture antibody is immobilized on the microtiter well in sandwich ELISA.

ELISA:

• The enzyme-linked immunosorbent assay is a widely used analytical biochemistry assay that was first described in 1971 by Eva Engvall and Peter Perlmann.
• The technique employs a solid-phase enzyme immunoassay to detect the presence of a ligand in a liquid sample, with antibodies directed against the protein under investigation.
• The Enzyme-Linked Immunosorbent Assay (ELISA) is an immunoassay test used to detect illnesses.
• The antigen-antibody interaction principle underpins ELISA.
• In this case, the particular antibodies connect with or bind to the target antigen.
• An antibody is a protein that the body's immune system produces when it recognizes dangerous chemicals known as antigens.

In ELISA, what is a microtiter plate?

• ELISA microplates are suited for biochemical and cell-based protein identification and quantification experiments.
• ELISA products can be found in a variety of immunodiagnostic and DNA-based diagnostic test kits.
• ELISAs are commonly done in polystyrene plates with 96 or 384 wells, which passively bind antibodies and proteins.
• Because of this binding and immobilization of chemicals, ELISAs are simple to develop and perform.

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