Question

Which one of the following BLAST search programs is used to identify homologs of a
genomic DNA query in a protein sequence database?
(A) blastp (B) blastn (C) blastx (D) tblastn

Answer 1

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Option.A

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Answer 2

Answer:

Blastx search program is used to identify homologs of a genomic DNA query in a protein sequence database.

Explanation:

BLAST (basic local alignment search tool) is an algorithmic tool for comparison of amino acid sequences, nucleotide sequences of DNA and RNA.  BLAST is very popular tool used for sequence searching. Various types of BLAST tools are available based on query sequences used for search.  

Nucleotide-nucleotide BLAST (blastn)

Protein-protein BLAST (blastp)

Nucleotide 6-frame translation-protein BLAST (blastx)

Nucleotide 6-frame translation-nucleotide 6-frame translation (tblastx)

Protein-nucleotide 6-frame translation (tblastn)

Position-specific Iterative BLAST (PSI-BLAST)

Large number of query sequences (megablast)

BLASTX uses translated nucleotide sequences to search protein database. It compares the 6 frame translation product of a nucleotide sequence from both strands against a protein sequence database.