Why cell wall less bacteria is not affdcted with lysozyme?
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L-forms can be generated in the laboratory from many bacterial species that usually have cell walls, such as Bacillus subtilis or Escherichia coli. This is done by inhibiting peptidoglycan synthesis with antibiotics or treating the cells with lysozyme, an enzyme that digests cell walls.
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Gram-positive bacteria are more susceptible to the action of lysozyme because their cell wall contains up to 90% peptidoglycan, whereas Gram-negative bacteria are more resistant because of the smaller amount of peptidoglycan in their cell wall.
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