Question

Why circular plasmid runs differently than a limearized plasmid on a gel?

Answer 1

The supercoiled form of plasmid DNA sustains less friction when run in agarose gel matrix. Therefore, for the same size plasmid DNA, supercoiled DNA form will runs faster than open circular form and linear form. ... Therefore, an uncut plasmid produces a complex banding pattern on an agarose gel.

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