Chemistry, asked by krautmahesh5421, 1 year ago

Why do we use ammonium sulphate for protein precipitation?

Answers

Answered by angel881
10

Answer:

Proteins are usually stored in ammonium sulfate because it inhibits bacterial growth. ... As the salt concentration is further increased, the solubility of the protein begins to decrease. At a sufficiently high ionic strength, the protein will precipitate out of the solution, an effect termed "salting out".

Answered by mohdshahil841
0

Answer:

Explanation:

Use a mortar and pestle to break up clumps and otherwise grind up the ammonium sulfate to facilitate addition and dissolution.

Add only a small amount of ammonium sulfate at a time, wait for it to dissolve and gently stir to avoid foaming.

Use a buffer such as 50 mM HEPES or Tris to minimize the acidifying nature of ammonium sulfate.

Use analytical grade ammonium sulfate since lower grades typically exhibit heavy metal contamination.

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