Question

why use wave length 450nm in ELISA techniques

Answer 1

Dont know the answer sorry...

Answer 2

I am trying to measure the amount of total protein dissolved in my solubilization buffer. I saw on paper that by measuring absorbance at 450 nm the turbidity of sample could be measured but I've heard before that for assessing turbidity the absorbance at 600 nm should be considered. What's the difference of these two absorbances? Which one is more proper for my goal?