why we apply ethanol in valigated leaf
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This procedure kills a leaf, disrupts the cell membranes and softens the cuticle and cell walls. This makes it possible to extract the chlorophyll with hot ethanol and also allows the iodine solution to penetrate the cells and react with any starch present.
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Ethanol is also known as alcohol.The hot water kills the leaf and the alcohol breaks down the chlorophyll, taking the green color out of the leaf.
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