Advantage and disadvantage of microinjection method
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Advantages
The amount of DNA delivered per cell is not limited by the technique and can be
optimized. This improves the chance for integrative transformation
The delivery is precise, again increasing the chance of integrative transformation.
The small structures can be injected containing only a few cells and with high regeneration potential.
Since it is a direct physical approach, it is host-range independent.
Disadvantages
Injection can cause damage that affects embryonic survival and can result in quite high mortalities.
Only one cell is targeted per injection
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