Cell lysis methods to obtain protein by physical and chemical methods
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Physical and mechanical methods of cell disruption have traditionally been the method of choice for cell lysis for two good reasons:
1) it can be used with a wide variety of meterials
2). The amount of force applied and duration of the treatment can easily be adjusted to suit the force meterial
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Some of the most commonly used chemicals for cell lysis include organic solvents (e.g., alcohols, ether, and chloroform), chelating agents (EDTA), detergents or surfactants (e.g., SDS, Triton) and chaotropic agents (e.g., urea, guanidine).
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