Biology, asked by kaushlendravij7764, 1 year ago

Give working and applications of uv-vis spectrophotometer.

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Answered by muskanc918

Ultraviolet and visible (UV-Vis) absorption spectroscopy is the measurement of the attenuation of a beam of light after it passes through a sample or after reflection from a sample surface. Absorption measurements can be at a single wavelength or over an extended spectral range.

UV-Vis is a fast, simple and inexpensive method to determine the concentration of an analyte in solution. ... In UV-Vis, a beam with a wavelength varying between 180 and 1100 nm passes through a solution in a cuvette. The sample in the cuvette absorbs this UV or visible radiation.

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