Question

If pBR322 cloning vector is cut by Pst I enzyme for
formation of r-DNA then which of its marker gene
helps in selection of recombinants ?
(1) amp^R gene (2) tet^R gene
(3) gal gene
(4) Both (1) and (2)​

Answer 1

Answer:

second lg rha he. Bhai.

Answer 2

Answer: The correct answer is both 1 and 2

Explanation: pBR322 is considered the first-ever cloning vector used to form recombinant DNA and it has two selectable markers already present which are tetracycline and ampicillin-resistant genes whereas the ampicillin has two restriction sites which are the ones where restriction enzyme recognize the sequence and cuts it and those two are pst1 and Pov 1 so if it is cut by Pst 1 then ampicillin will be inactive due to insertional inactivation of that gene but tetracycline helps to tell they are non reombinant hence both 1 and 2.

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