Question

List the steps followed in DNA fingerprinting technique.

Answer 1

it uses southern blot hybridization using radio labelled VNTR as a probe. Its steps include :

i) Isolation of DNA

ii)Digestion of DNA by restriction endonucleases.

iii)Separation of DNA fragments by electrophosis.

iv)Blotting of separated DNA fragments to synthetic membranes, nitro cellulose or nylon.

HOPE THIS HELPS YOU.

Answer 2

What is DNA Fingerprinting?

=> A chemical test that shows the genetic makeup of a person or other living things.

Steps of DNA Fingerprint (Southern Blotting techniques):

1. DNA extract from cell.
2. Cutting DNA by restrictions Endonuclease.
3. Separate DNA fragments on Electrophoresis gel.
4. Transfer DNA onto paper.
5. Adding the radioactive probe.
6. Setting up the X-ray film.