Question

what is DNA finger printing ?
explain in brief but in points
where is this technique used ?
give two examples and have a brainly mark for the whole answer

Answer 1

HEY LEARNER,

Here is ur answer,

DNA Finger printing :

• It is the technique to compare the DNA fragments of two individuals.
• Developed by Alec Jeffreys.

Steps of DNA Finger printing:

• Isolate DNA ( from any cells like blood stains , semen or hair roots ).
• Make copies ( amplification) of DNA  by Polymerase Chain Reaction (PCR) if the amount of isolated DNA is small.
• Digest DNA by restriction endonucleases.
• Separate DNA fragments by gel electrophoresis over agarose polymer gel .
• Treat with alkali solution ( NaOH) to denature DNA bonds so as to split them into single stranded DNAs in the the gel .
• Transfer single stranded DNA fragments to synthetic membranes sych as nitrocellulose or nylon , and then baked in a vacuum oven at 80 degree celcius for 3-5 hours ( to fix the DNA fragment on the chromosome).
• Nitrocellulose filter membrane is placed in a solution containing radio active labelled single stranded DNA probe. The DNA probes are small radio active synthetic DNA segments of known sequences of nitrogen bases. These DNA probe binds with the complementary sequences of the DNA fragment on the membrane to form a hybridized DNA.
• The filter paper is washed to remove unbound probe.
• The hybridized Dna is photographed on to an X ray film by autoradiography. The image ( in the form of dark and light bands ) obtained is called DNA fingerprint . This gives the characteristic pattern of individual's DNA.

Applications  :

• For the diagnosis of genetic disorders
• Forsenic tool to solve paternity , murder , etc.

HOPE IT HELPS U!!!

Answer 2

Answer:

DNA fingerprinting :

1) DNA sequence of each person is unique as that of the fingerprinting .

2)Due to this , identity of any person can be established with the help of its available DNA .

ETC.,

TECHNIQUE USED :

1)CRIMINAL SITE