Question

what procedure did you follow to observe lactobacillus bacteria in the lab

Answer 1

Lactobacillus is a most commonly found bacteria in milk, yogurt and other dairy products.

They are rod shaped, non-spore forming, gram positive bacteria.

We can observe them by making  a slide of yogurt:

-Take a glass slide and put a drop of diluted yogurt on it, make a smear for 2 to 3 secs on the slide.

-Add a drop of methylene blue on the slide.

-Cover the slide with a cover slip.

-Place it under the compound microscope

- You will observe the rod shaped lactobacilli in it.

Answer 2

Answer

1. Take one or two drops of butter milk on a slide and spread it.

2. Heating the slide gently on a lame.

3. Add a few drops of crystal violet and leave it for 30 to 60 seconds.

4. Then wash the slide gently with water and observe under the compound microscope.