steps of elisa test in simply
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ELISA Step-by-step :-
1). Antibody coating. Specific capture antibody is immobilized on high protein-binding plates by overnight incubation. ...
2). Protein capture. Samples and standard dilutions are added to the wells and will be captured by the bound antibodies.
3). Detection antibody. ...
4). Streptavidin-enzyme conjugate. ...
5). Addition of substrate. ...
6). Analysis.
1). Antibody coating. Specific capture antibody is immobilized on high protein-binding plates by overnight incubation. ...
2). Protein capture. Samples and standard dilutions are added to the wells and will be captured by the bound antibodies.
3). Detection antibody. ...
4). Streptavidin-enzyme conjugate. ...
5). Addition of substrate. ...
6). Analysis.
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