Question

Why selectable markers are essential in cloning vector?

Answer 1

Selectable markers are essential marker cloning vector to identify and eliminate non - transformants and selectively permitted the growth of transformants.

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Answer 2

Selectable Marker

• The vector also requires a selectable marker.

• It helps in identifying and eliminating non transformants by selectively permitting the growth of the transformants.

• A bacterium which has modified due to the introduction of a piece of foreign DNA is called transformant.

• The process through which a piece of DNA is introduced in a host bacterium is called transformation.

• Normally the genes encoding resistance to antibiotic such as ampicillin, trtracycline etc are considered useful selectable marker for E.coli.